THERMOSTABLE RESTRICTION ENDONUCLEASES FROM THERMOPHILIC BACTERIA
نویسندگان
چکیده
منابع مشابه
Mesophilic cyanobacteria producing thermophilic restriction endonucleases.
When searching for the site-specific endonucleases in several strains of Phormidium we made the following observations. Among the 16 strains that originated from 15 species of Phormidium, 12 produced one or more restriction enzymes, of which two produced the highly thermophilic restriction endonucleases PtaI and PpaAII with their optimum activity at 65-80 degrees C, which is far above the letha...
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There are three mechanisms by which foreign DNA may enter a prokaryotic organism: conjugation between Gram-negative bacteria, uptake of exogenous DNA (transformation) and infection by viral (bacteriophage) nucleic acid. The interaction between a bacteriophage and its host bacterial cell has been extensively investigated. A bacterium can restrict phage infection through its ability to degrade th...
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Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificit...
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Two new site-specific endonucleases, N1a III and N1a IV, have been isolated from Neisseria lactamica. N1a III recognizes the sequence, CATG, and cleaves 3' of the sequence to produce a four base 3' extension. N1a IV recognizes the sequence, GGNNCC, and cleaves between the two N's to produce blunt ended fragments.
متن کاملThermostable protease from thermophilic bacteria. II. Studies on the stability of the protease.
1. The effect of temperature on the conformation of the protease from Bacillus thermoproteolyticus was examined. 2. The thermostable enzyme is stable at room temperature against such denaturing reagents as urea, alcohol, and detergent. 3. However, these reagents accelerate the denaturation of the enzyme by heat. Materials-The crystalline protease of B. thermoproteolyticus was kindly supplied by...
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ژورنال
عنوان ژورنال: INTERNATIONAL RESEARCH JOURNAL OF PHARMACY
سال: 2014
ISSN: 2230-8407
DOI: 10.7897/2230-8407.050455